Peak Template Alignment

This video will demonstrate alignment using template matching in GC Image.

Note

Demos 1, 2, and 3 should be completed in sequence.

The public dataset used in this demo can be downloaded here:

• Benchmark GC×GC Data, Standards, Fragrances and Allergens

Supporting files to help in following along with this quick start demo can be downloaded here:

• Fragrances Demo Processing Files

1 Creating Templates

Templates allow us to match compounds between chromatograms, even if they are misaligned. The template created from this step is included in the Fragrances Demo Processing Files linked above (Fragrances.bt).

1. From the menu, select View > Blob Table.
2. From the blob table, select all blobs from both the Alkanes and Fragrances groups.
3. Click the Add to Template button on the blob table toolbar. Close the blob table.
4. From the toolbar, select the Template mode to view the newly added template peaks.
5. From the menu, select View > Template Table.
6. Click the Save Template button on the template table toolbar. Browse to a desired save location and click Save.

2 Matching Templates

We can use templates to quickly identify compounds of interest in similar samples.

1. Open a similar sample and process the image file using similar steps.
2. From the toolbar, select the Template mode.
3. From the menu, select Template > Load Template. Browse to the previously saved template and click Load.
4. Adjust the template matching settings with Configure > Settings. Select Template Matching from the list on the left.
   • Click the Defaults button.
   • Set the Col I (pixels) Distance Threshold to 4.
   • Enable both Match Factor and Reverse Match Factor Spectral Constraints and set both values to 700.
   • Click OK to save the settings.
5. From the menu, select Template > Match Template.
6. Review the displayed Matching Summary report, then close the dialog.
7. Open the Blob Table in order to see the see the results of the next step.
8. From the menu, select Template > Apply Template.