How to handle No-Reliable-Peak failure when generating a feature template

qtao · Post by **qtao** » July 20th, 2022, 11:20 am

Question: A No-Reliable-Peak error occurs when trying to
use the Auto Feature option with the Investigator/Analysis program to generate a feature template for multiple samples. What’s going wrong and how to fix it?

Answer:
The problem is because the software failed to find any reliable peaks that are required for retention time alignment. In general, possible reasons might be:

There are some chromatograms that are way different from others.
Too many background noise regions were detected as peaks on individual chromatograms.
Template Matching settings are not appropriate, for example, Configure > Settings > Template > Auto CLIC > Match Factor, and Template Matching > Distance Thresholds

If you are not sure about the reason, you may try to set the number of Preferred Reliable Peaks and update the feature model folder (.gcfm) as follows:

Delete the "Reliable" folder under the gcfm folder
File > Load Images > Choose the exact same set of image files
Choose Auto Feature, and the same gcfm folder so that the software can restart where it fails instead of re-processing
Specify Preferred Reliable Peaks
OK and choose to Update