Hello,
I deal with a certain problem for a longer period of time now.
I do my measurements with GCxGC-QTOF/MS and I am not able to open different MSMS data with GC image. In my method there are different time segments where I have different precursor ions. In the 2D chromatogram I can see that the MSMS method works but I am not able to open it in GC Image.
It would be really helpful to get a guidance how to open different types of MSMS data.
Thank you for your help!
Import of MSMS data in GC Image
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- Posts: 236
- Joined: October 7th, 2011, 10:16 am
Re: Import of MSMS data in GC Image
Can you import the data and see a normal 2D TIC in GC Image?
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- Posts: 2
- Joined: June 28th, 2019, 7:26 am
Re: Import of MSMS data in GC Image
I don't see a 2D TIC in GC Image. I only see the 2D TIC in the MassHunter (Agilent) Software.
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- Posts: 236
- Joined: October 7th, 2011, 10:16 am
Re: Import of MSMS data in GC Image
Here is a quick check list:
- Does the program report any error when Import Image?
Check here for a MassHunter-related problem. - After Import, does the TIC look normal?
In order to generate a 2D chromatogram image, raw data need to be uniformly sampled. But almost any MS/MS mode results in non-uniform sampling. So you may need to turn on Advanced > Interpolation (either Linear or NN) with Auto Resampling in the Import dialog. More details on interpolation are discussed here.