GC Image LCxLC-HRMS Edition Users' Guide
New Features and Improvements in Release 2.7
GC Image LCxLC-HRMS Edition Release 2.7 (June 2017) has many new features and
improvements. See the GC Image LCxLC-HRMS Edition
Users' Guide for full documentation. GC Image LCxLC-HRMS Edition R2.7
requires Microsoft Windows Vista or later operating system.
GC Image LCxLC-HRMS Edition R2.7 (64-bit) requires a 64-bit Microsoft Windows operating
system. The software requires Microsoft .Net Framework 4.0 or later to
import some vendor data file formats.
Improved Agilent 2D-LC Data Support
GC Image LCxLC-HRMS Edition R2.7 now can utilize the Agilent 2D-LC specific
metadata to import multiple heart-cutting, high-resolution sampling (i.e. selective), or comprehensive
2D-LC data, and construct a 2D chromatogram automatically.
Large Data File Support
GC Image LCxLC-HRMS Edition R2.7 includes significant improvements for reading and writing
large CDF files, which are used as the file store for MS data.
- The software now supports reading and writing of
larger CDF files that can contain more data than previously allowed.
The maximum data size is now only limited by the file
system and memory of the computer.
- Additional improvements yield faster read times for
CDF files on hard disk drives. LC Image now can open
large CDF files much faster!
Mass Spectra Centroiding, Calibration, and Viewing
GC Image LCxLC-HRMS Edition R2.7 has new and improved operations for
mass calibration with HRMS data, including:
- The software supports exporting centroid data
extracted from profile data directly to a GCI file (with accompanying CDF
file), in addition to the option of exporting to a standalone CDF file.
- The software supports recalibrating centroid data
with or without profile data, in addition to recalibrating the profile data only.
After recalibration is configured, users can export
the recalibrated centroid data.
- The software has a new, default, context-based MS mode
that opens MS View for point, blob, or area spectra based on content.
If the mouse-click location is not within a blob or area, a point
spectrum is opened; otherwise, both the apex and summed spectra
for the blob or area are opened. If the location is within more
than one object, a popup menu allows selection.
MS Library Search for Area Objects
GC Image LCxLC-HRMS Edition R2.7 introduces MS library search supports for areas.
- New Library Search actions directly search
for matches with spectra from all or selected areas.
- MS Viewer now can apply the hit list from a manual library search to
- Area properties now include a Hit List that allows
users to examine search results and select the preferred match.
- Library properties now can be added to the Areas
tab of the Blob Table.
New Quantifier Ion Properties
GC Image LCxLC-HRMS Edition R2.7 has new properties and improved
operations for quantifier ions, including:
- A quantifier ion can be set as an internal standard for blobs and
areas for calibration and quantification, to support
isotope dilution analysis.
- New blob/area statistics for quantifier ions include retention
times, area, peak value, and volume.
New Calibration and Quantification Tools
GC Image LCxLC-HRMS Edition R2.7 introduces new tools for calibration
and quantification. The process for generating calibration tables is
simplified by a new UI and options, including:
- New Quantification
menu options for tools to conveniently create calibration tables
directly from a set of chromatograms, and to load, edit, and save calibration tables.
- A new Calibration Table interface that allows users to examine
and edit individual calibrants. Calibration points and graphs of
the calibration curves are easily accessible through a tabbed view.
- New calibration function types, Quadratic and Piecewise Linear,
in addition to Linear.
- New calibration method files that allow calibration settings
to be customized and stored for reuse.
- Quantitation using ISTD Ions have been made easily available with the new
quantitation settings and UI improvements.
- Additional quantitation settings have been added to give users more
options. The quantification settings allow users to control the
targets to be quantified, how to measure the quantifier ions, the
uncalibrated response type, and if they want to allow extrapolation.
GC Image™ LCxLC-HRMS Edition Users' Guide © 2001–2017 by GC Image, LLC, and the
University of Nebraska.