- Algorithm A: Gros, J., Nabi, D., Dimitriou-Christidis, P., Rutler, R., Arey, J. S., “Robust algorithm for aligning two-dimensional chromatograms”, Anal. Chem. 2012, vol 84, p 9033-9040.
- Algorithm B: Zushi, Y., Gros, J., Tao, Q., Reichenbach, S. E., Hashimoto, S., Arey, J. S., “Pixel-by-pixel correction of retention time shifts in chromatograms from comprehensive two-dimensional gas chromatography coupled to high resolution time-of-flight mass spectrometry”, Journal of Chromatography A 2017, vol 28, p 121-129.
The NIES-EPFL Transform plugin requires Version 2.6 or later.
- Download here: the NIES-EPFL Transform plugin
- Unzip it using File Explorer or another application.
- Start GC Image.
- From the menu, select Tools > Manage Plugins...
- Click on Import and then select the NIES-Alignment.plugin folder.
- Press OK to finish the installation. NIES-EPFL Transform will now be listed in the table.
To align chromatograms using NIES-EPFL Transform, select Processing > Register Image... in Image or Run > Register Images in Project. The NIES-EPFL Transform option will become available in the Transform Type combo-box. More details related to the Register Image action can be found in the User Guide.
The plugin can be configured to perform either of the two original methods as follows:
- Algorithm A for non-MS data
- Choose to use Bicubic as the Interpolation Type.
- Algorithm B for MS and HRMS data
- For HRMS data, choose to use Nearest Neighbor as the Interpolation Type.
- For MS data, from Configure > Settings > Multi-Channel select By Integer Intervals for the MS Data Binning, and choose to use Bilinear as the Interpolation Type.
- Typical peak widths (Col I and Col II) in pixel units, which defaults to 3 and 120 pixels.
- The deformation correction enabled flag in true or false, which defaults to true.