Reference MS

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michaelgroessl
Posts: 7
Joined: February 2nd, 2017, 7:40 am

Reference MS

Post by michaelgroessl » March 6th, 2017, 9:47 am

When applying a template to a blob table, the reference MS filed in Blob Properties/Analysis is filled automatically. What does the reference MS refer to?

qtao
Posts: 156
Joined: October 7th, 2011, 10:16 am

Re: Reference MS

Post by qtao » March 6th, 2017, 10:08 am

Reference MS is added when adding a blob to template. It is the spectrum of the blob, either peak (apex) or blob(integrated) MS, which depends on the following setting:

Configure > Settings > Library Search > Type of Spectrum

michaelgroessl
Posts: 7
Joined: February 2nd, 2017, 7:40 am

Re: Reference MS

Post by michaelgroessl » March 6th, 2017, 10:20 am

Thanks.
So is this reference MS of the template somehow matched to blobs when clicking "match template"? If so, how is this done - is there some type of similarity score calculated? Is is possible to manually compare the spectrum of the template to the blob spectrum?

qtao
Posts: 156
Joined: October 7th, 2011, 10:16 am

Re: Reference MS

Post by qtao » March 6th, 2017, 12:26 pm

You need to set Qualifier CLIC to use Reference MS, for example,

Code: Select all

Match("<ms>") >= 800.0
You can let the program generate it automatically whenever adding a blob to template by Configure > Settings > Template > Auto CLIC > check Create Qualifier CLIC.... You can find more details here.

There is no visual comparison between Reference and Blob spectra. But you can compute the match factors between them after applying template by setting Analysis CLIC to

Code: Select all

Match("<ms>") 
, then add aCLIC Result column to Blob Table.

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